In Vitro Metabolism of T-2 Toxin
نویسندگان
چکیده
منابع مشابه
In vitro metabolism of T-2 toxin.
Incubation of T-2 toxin with the 9,000 x g supernatant fluid of both human and bovine liver homogenate resulted in conversion to a single, deacetylated product identified as HT-2 toxin. Metabolism is more rapid in human liver. HT-2 toxin was not produced when human plasma was the incubating medium nor was it produced by treatment of T-2 toxin with simulated gastric juice. T-2 toxin was stable i...
متن کاملIn vitro metabolism of T-2 toxin in rats.
T-2 toxin was rapidly converted in the 9,000 X g supernatant fraction of rat liver homogenate into HT-2 toxin, T-2 tetraol, and two unknown metabolites designated as TMR-1 and TMR-2. TMR-1 was characterized as 4-deacetylneosolaniol (15-acetoxy-3 alpha, 4 beta, 8 alpha-trihydroxy-12,13-epoxytrichothec-9-ene) by spectroscopic analyses. Since the same metabolites were also obtained from HT-2 toxin...
متن کاملThe roles of carboxylesterase and CYP isozymes on the in vitro metabolism of T-2 toxin
BACKGROUND T-2 toxin poses a great threat to human health because it has the highest toxicity of the currently known trichothecene mycotoxins. To understand the in vivo toxicity and transformation mechanism of T-2 toxin, we investigated the role of one kind of principal phase I drug-metabolizing enzymes (cytochrome P450 [CYP450] enzymes) on the metabolism of T-2 toxin, which are crucial to the ...
متن کاملModification of in vitro metabolism of T-2 toxin by esterase inhibitors.
In vitro metabolism of T-2 toxin with S-9 fraction obtained from livers of phenobarbital-treated pigs and rats in the presence of different esterase inhibitors, including NaF, p-hydroxymercuribenzoate, phenylmethylsulfonyl fluoride, eserine sulfate, diisopropylfluorophosphate, and diethyl p-nitrophenyl phosphate, was studied. The metabolism was completely shifted to the hydroxylation at the C-3...
متن کاملMetabolism of HT-2 Toxin and T-2 Toxin in Oats
The Fusarium mycotoxins HT-2 toxin (HT2) and T-2 toxin (T2) are frequent contaminants in oats. These toxins, but also their plant metabolites, may contribute to toxicological effects. This work describes the use of 13C-assisted liquid chromatography-high-resolution mass spectrometry for the first comprehensive study on the biotransformation of HT2 and T2 in oats. Using this approach, 16 HT2 and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1974
ISSN: 0003-6919
DOI: 10.1128/am.27.2.423-424.1974